Exhaled Nitric Oxide Analyzer
MULTI-GAS BREATH
ANALYSIS FOR
CLINICAL & VETERINARY
RESEARCH
The DLSIB measures exhaled nitric oxide, oxygen, carbon dioxide, airflow, temperature, and humidity in a single synchronized recording. Designed for respiratory researchers and equine veterinarians who need correlated multi-gas data from every breath.
WHAT THE DLSIB MEASURES
Six measurement channels operate simultaneously through a shared airway, producing time-aligned data at configurable sample rates up to 100 Hz. Every channel is calibrated individually and logged with millisecond-resolution timestamps.
Exhaled Nitric Oxide (NO)
ppb-level sensitivity
Electrochemical sensor with dedicated potentiostat and transimpedance amplifier. Temperature-compensated baseline correction eliminates drift across extended recordings.
Oxygen (O2)
0–25% v/v range
Galvanic cell with transimpedance amplifier readout. Linear calibration validated against certified reference gases to R² > 0.999.
Carbon Dioxide (CO2)
0–50,000 ppm
Non-dispersive infrared (NDIR) module with in-situ verification. Published calibration achieves R² = 0.9999 across full capnographic range.
Mass Airflow
Bidirectional tidal flow
Hot-wire mass airflow sensor captures inspiratory and expiratory volumes per breath cycle, enabling real-time spirometric analysis alongside gas concentrations.
Temperature
Breath & ambient
Precision thermistor tracks exhaled breath temperature for BTPS correction and provides environmental context for gas sensor compensation.
Relative Humidity
Continuous monitoring
Capacitive humidity sensor logs moisture content throughout the recording, critical for accurate gas concentration calculations in saturated exhaled air.
WHO USES MULTI-GAS
BREATH ANALYSIS
The DLSIB was developed at Texas Tech University for researchers and clinicians who need more than a single-gas snapshot. Three areas drive the strongest demand.
Respiratory Research
Exhaled nitric oxide is an established biomarker for eosinophilic airway inflammation, but its diagnostic value increases substantially when correlated with CO2 waveform shape, O2 extraction ratio, and tidal volume. The DLSIB captures all of these in one recording, giving researchers a multi-dimensional view of pulmonary function that single-channel FeNO analyzers cannot provide. Ideal for longitudinal asthma studies, occupational exposure monitoring, and therapeutic response tracking.
Equine Respiratory Diagnostics
Inflammatory airway disease and exercise-induced pulmonary hemorrhage are costly conditions in performance horses, yet current diagnosis typically requires bronchoalveolar lavage under sedation. The DLSIB enables non-invasive breath screening at the barn or trackside. Developed in collaboration with the Texas Tech School of Veterinary Medicine, the portable unit connects to equine-compatible masks for field collection, reducing animal stress while producing quantitative gas profiles that can be tracked across training seasons.
Early Disease Detection
Subtle shifts in exhaled gas ratios can precede clinical symptoms by weeks or months. Elevated NO alongside abnormal CO2 capnography patterns may signal developing airway obstruction before spirometry alone would flag it. The DLSIB's synchronized, multi-gas data logging makes it possible to build baseline profiles and detect deviations early, supporting preventive intervention in occupational health programs, athletic screening, and chronic disease management.
WHY SYNCHRONIZED MULTI-GAS
MEASUREMENT MATTERS
Single-gas analyzers measure one parameter at a time. That approach discards the temporal correlations between gases that often carry the most diagnostic information.
- close Separate devices for FeNO, capnography, and spirometry
- close Measurements taken minutes or hours apart under varying conditions
- close Cross-parameter correlations lost because data is not time-aligned
- close Combined equipment cost often exceeds $30,000
- check_circle All six channels sampled from the same breath at the same moment
- check_circle Millisecond-aligned timestamps reveal how NO, CO2, and airflow interact within each breath cycle
- check_circle Temperature and humidity logged continuously for accurate BTPS and gas correction
- check_circle One portable device at a fraction of the cost of multi-instrument setups
Data Logging Architecture
The DLSIB uses a mixed-signal data-logging architecture with onboard 4 Mb EEPROM and a time/date-stamped file system. Data streams to the companion GUI over USB for real-time synchronized strip-chart display, and can also be exported as CSV for integration with MATLAB, Python, R, or any analysis pipeline. Adjustable sample rates and per-unit calibration profiles ensure repeatability across instruments and recording sessions. Wireless connectivity via Bluetooth, Wi-Fi, or LoRaWAN is supported for remote and field deployments.
BREATH ANALYZER Q&A
What gases does the BSI breath analyzer measure?
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The DLSIB simultaneously measures exhaled nitric oxide (NO) at parts-per-billion sensitivity, oxygen (O2) across 0–25% v/v, and carbon dioxide (CO2) from 0 to 50,000 ppm using NDIR spectroscopy. It also records bidirectional mass airflow, breath temperature, and relative humidity. All six channels are time-synchronized, producing a complete respiratory profile from a single recording session.
Can the DLSIB be used for equine respiratory diagnostics?
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Yes. The system was developed in part through collaboration with the Texas Tech School of Veterinary Medicine specifically for equine respiratory assessment. It connects to equine-compatible collection masks for non-invasive field screening. This approach can supplement or reduce reliance on bronchoalveolar lavage, lowering animal stress while providing quantitative, repeatable gas concentration data that can be tracked across seasons or treatment periods.
How is exhaled nitric oxide measured in the DLSIB?
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Exhaled NO is detected by an electrochemical sensor connected to a custom potentiostat and transimpedance amplifier circuit designed by BSI. The analog front-end converts the sensor's picoamp-level oxidation current into a digitizable voltage. Temperature-compensated baseline correction runs continuously to eliminate thermal drift, enabling stable ppb-level measurements over extended recording periods. The sensor's calibration coefficients are stored per-unit for instrument-to-instrument consistency.
Is the analyzer portable enough for field use?
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The DLSIB was designed from the ground up for field deployment. It operates on battery or wall power, stores recordings in onboard EEPROM with a time/date-stamped file system, and supports Bluetooth, Wi-Fi, and LoRaWAN for wireless data transfer. The unit is compact enough to carry to a barn, trackside, or remote clinical site. No external computer is required during data collection, though the companion GUI software provides real-time visualization when a laptop is available.
What data formats does the DLSIB support?
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All recordings export as CSV files with millisecond-resolution timestamps, making them directly compatible with MATLAB, Python, R, Excel, and custom analysis pipelines. The companion GUI application also provides real-time synchronized strip-chart visualization of all channels and includes a calibration utility with automated curve fitting from linear through fourth-order polynomial models. Each instrument's calibration profiles are stored by serial number for traceability.
READY TO EXPLORE
MULTI-GAS BREATH ANALYSIS?
Whether you are planning a respiratory research study, evaluating equine diagnostic workflows, or exploring early disease detection, we can help you determine how synchronized multi-gas measurement fits your protocol.